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Recombinant Anti- HIS Tag Mouse mAb

價格: 在線咨詢
貨號:
DRF10140
產(chǎn)品規(guī)格:
100 μL
Applications:
WB
Species:
購買數(shù)量:
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Product Information


Description

6 X His Tag mouse monoclonal antibody

Synonyms

6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED

Immunogen

Synthetic peptide HHHHHH (6* His)

Isotype

IgG

Purity

Affinity purification 




Applications


Applications

Dilution

Positive Tissue

WB

1: 2000-1: 5000

His-Tag fusion protein




Background


Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.




Images


Western blot analysis of His Tag (GB151251) 

at dilution of 1: 5000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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