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Anti -MCM2 Rabbit pAb

價(jià)格: 在線咨詢
貨號(hào):
DRF101287
產(chǎn)品規(guī)格:
100 μL
Applications:
WB | IHC | IF
Species:
H,M,R | H,M,R | H,M,R
購買數(shù)量:
產(chǎn)品詳情 操作說明 注意事項(xiàng)

Product Information


Description

MCM2 rabbit polyclonal

Protein full name

DNA replication licensing factor MCM2

Synonyms

Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, Mcm2, Bm28, Cdcl1, Kiaa0030, Mcmd2

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse MCM2

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW.

102 kDa

Observed MW.

130 kDa

Uniprot ID

P49736, P97310, D3ZP96




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

thymus

IHC

Human, Mouse, Rat

1: 1000-1: 4000

lung cancer, brain, colon cancer, thymus, lung

IF

Human, Mouse, Rat

1: 1000-1: 3000

brain, lung, thymus




Background


The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.




Images


Western blot analysis of MCM2 (GB111162) at dilution of 1: 1000
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: MCF7 cell lysate
Lane 4: SW480 cell lysate
Lane 5: Raji cell lysate
Lane 6: Mouse thymus tissue lysate
Lane 7: Rat thymus tissue lysate

Immunohistochemistry analysis of paraffin-embedded human lung cancer using MCM2 (GB111162) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded human colon cancer using MCM2 (GB111162) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded mouse brain using MCM2 (GB111162) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded mouse thymus using MCM2 (GB111162) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded rat thymus using MCM2 (GB111162) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded rat lung using MCM2 (GB111162) at dilution of 1: 2000

Immunofluorescent analysis of paraffin-embedded mouse brain using MCM2 (GB111162) at dilution of 1: 2000

Immunofluorescent analysis of paraffin-embedded mouse lung using MCM2 (GB111162) at dilution of 1: 2000

Immunofluorescent analysis of paraffin-embedded rat thymus using MCM2 (GB111162) at dilution of 1: 2000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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